Techne Prime Pro 48 Real-time qPCR machine, 100-240V

Description

Techne Prime Pro 48 Real-time qPCR machine, 100-240V
The Prime Pro 48 real time PCR system is a high specification, economically priced real time thermal cycler that accommodates a unique 48-well polypropylene PCR plate utilising the same geometry as standard 384-well plates, but only 1/8 of the size. This enables users to dramatically reduce the qPCR reagent volumes compared to traditional 96-well instruments, saving users precious sample, whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity. A minimum volume of 5μl is validated, resulting in a more efficient use of expensive and ‘hard to acquire‘ template DNA samples.
The Prime Pro 48 real time qPCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.
High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimised 40 cycle reaction down to only 15 minutes. qPCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.
The capacity of Prime Pro 48 accommodates a unique and economical 48-well qPCR plate. The well format mirrors a 384-well plate therefore allowing the use of 16-channel pipettes. The small qPCR plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity and greatly reduces run times.
Key features
Speed
40 cycles in 40 minutes before optimisation.
40 cycles in 15 minutes when optimised.
Genotype in 4 minutes with over 99% accuracy.
Set up a 4plex reaction in only 4 mouse clicks.
Confidence
MIQE compliant - always consistent, always compliant.
Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system.
400 analyte specific qPCR reagent kits available.
Demo equipment available to evaluate in your own lab.
Performance
Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine.
Compile data from multiple experiments. Pool plates and results across labs and instruments.
Record every well, with every filter, in every cycle. Never miss a data point.
Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighbouring wells or maximising the wide dynamic range. Trust results are correct.
All other block-based qPCR machines have lower uniformity. If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications.
Value
No other qPCR system offers so much performance for such a low price.
Available as a complete system - Techne instrument, plates, seals and reagents.
Alternatively, is chemically open platform - use your preferred supplier reagents.
Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
Everything as standard - no add-ons, HRM as standard, unlimited software licence.
Highly accurate and uniform qPCR prevents means successful NGS runs and significant money saving.
Sensitivity
±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
HRM - Identify Class IV SNP >99.9% of the time
Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines.
Load samples into 48 well plate using backlit loading block.
Insert plate into Prime Pro 48.
Run a MIQE compliant experiment via ProControl software.
Analyse results in ProStudy software.
Generate a full report automatically.
The ergonomic plate loading dock allow for easy loading of the plate and is backlit to allow the user to easily visualise sample loading.
Base with LED backlight
Plate adapter fits standard centrifuge rotors
Includes plate sealing tool
Advanced optical technology
Intuitive icons lead researchers through setup, run and analysis quickly and easily
Sensitive optical system delivers precise detection for a range of fluorophores
Convenient 48-well format meets the throughput needs of most researchers
Unique thermal system provides unmatched temperature control for the most accurate results
The Pro 48 Real-time system offers the qPCR capabilities of larger instruments in a compact, accurate footprint. Innovative features include a precise thermal system for unrivalled temperature control, an advanced optical system for highly sensitive fluorescence detection, a 48-well plate for flexible sample throughput, and intuitive, icon-driven software for error-free instrument operation.
Prime Pro 48 thermal system
qPCR specificity and efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the entire heat block, ensuring that all samples are processed equally. The unique thermal block design of Pro 48 achieves this with a unique heating and cooling system that provides accurate ±0.1ºC temperature control and quickly cycles from one temperature to the next.
To achieve true temperature control the Pro 48 thermal system consists of a precisely electro formed 48-well hollow silver block containing conductive fluid. The block is heated and cooled by a single peltier device, with an agitator assembly consisting of two paddles driven by electromagnetic motors. During PCR cycling, the paddles move rapidly, circulating the fluid across the 48 wells, allowing the block to achieve high ramp rates, thus reducing the overall experiment time.
This unique design delivers industry leading thermal stability of ±0.1°C virtually eliminating thermal non-uniformity and preventing edge effect. The result is higher qPCR performance, tighter Cq, greater PCR efficiency, higher R 2 and the ability to perform demanding HRM applications.
Fast, uniform temperature control is important because accurate dwell temperatures ensure primers bind most efficiently and polymerase enzymes work optimally, generating the maximum yield of target DNA. The hermetically sealed hollow block contains a conductive fluid and two opposing agitators driven by electromagnetic motors. During PCR cycling, these agitators rapidly circulate fluid throughout the hollow block, transferring heat from the peltier quickly and evenly.
Thermal system
The Prime Pro 48 Real Time PCR system contains an advanced high-performance optical system that delivers precise and sensitive fluorescence detection, facilitating all 4 colour multiplex applications. The system is provided factory-calibrated for use with SYBR, FAM, HEX, VIC, ROX, Cy 5 and can also be used with any qPCR fluorophores overlapping with these calibrated dyes. For excitation, two panels of 48 fixed LEDs provide excitation energy of distinct spectra, enabling broad range excitation. Each of the 48 LEDs illuminates a specific well location, eliminating the optical distortion created by most stationary optical systems. HRM analysis protocols are supported by continuous data acquisition during the melt for increased data collection and reduced run times, HRM of a full plate is less than 10 minutes. The user can change the plate setup and perform data analysis after the run is complete.
Adaptive LED control (ALC)
ALC normalises variation in fluorescence across all wells at each cycle of a run and provides specific tuning for each LED following each PCR cycle.
Speeds up data collection because only 1 image is required to cover the full sample dynamic range.
Expands the linear range of detection by reducing LED exposure to high emission wells and preventing premature detector saturation.
LED adjustment following each amplification allows Prime Pro 48 to reduce exposure time as signal intensity increases, maximizing the linear range of emission detection.
Minimize optical artifacts by keeping each well brightness similar to the CCD. This reduces the influence of a highly fluorescing sample on adjacent wells and avoids ‘blooming’, no artifacts from adjacent wells. • Maximize sensitivity by permitting an appropriate exposure level for each well, by dye, rather than compromise with a universal setting.
Applications
The Prime Pro 48 is a high performance instrument for demanding applications.
Absolute & Relative Quantitation
Absolute quantification standard curves with efficiency calculation.
Relative quantification using the ΔΔCq method with support for multiple reference gene normalization.
Relative gene expression with efficiency control.
Next Generation Sequencing (NGS) library quantification.
Copy Number Variation: insertions, deletions, inversions.
RNA Characterisation: siRNA and miRNA.
Viral load.
Genotyping and High resolution melting
Allelic Discrimination.
DNA Methylation.
SNP Verification.
Microbiology: pathogen detection and identification.
High resolution melting (HRM)
Genotyping, methylation studies and mutation screening.
Library quantification
qPCR is the most precise method for quantifying libraries prior to cluster generation.
Works at concentrations below the detection threshold of conventional spectrophotometric methods.
Data
Validation of array and sequencing quality data.
Design for:
Academic, Government and Corporate laboratories.
Human research, AgriGenomics, Translational, Forensics and many more markets.
Probe, DNA Binding Dye and HRM chemistries.
Gene expression, Viral detection, Sequencing Library Preparation, Genotyping and Mutation Screening applications.
Pro 48 is for research use only – not suitable for human or veterinary clinical diagnostics.
Rapid cycling
The Pro 48 has rapid heating and cooling rates. Average
ramp rates of 5.5°C/sec reduce overall PCR run times.
The typical run time for a 40 cycle PCR protocol is under
20 minutes when optimised.
High throughput
Pro 48 thermal control is superior to traditional 96-well peltier systems where thermal efficiency decreases as distance from the peltier increases. With Pro 48, faster and more precise cycling increases sample throughput because the need for multiple replicates is reduced by increased uniformity across the block. Pro 48 well-to-well, plate-to-plate and instrument-to-instrument results are as consistent as running a single experiment. To take advantage of this, Pro 48 has a function in the software where multiple experiments are combined. High uniformity eliminates the need for triplicates which reduces reagent running costs and ensures the entire plate of data is valuable.
Software
Easy to use software that integrates user control, real-time data collection, and advanced data analysis.
Pro software
User-installable, intuitive, easy to use software that integrates user control, real-time data collection, and advanced data analysis.
Supplied fully featured including HRM capability.
New software version 5 enables a widened chemistry library and contains new thermal profiles.
Facilitates rapid thermal cycling: 40 cycles in 40 minutes.
Supplied on a USB stick.
ProStudy software
Data analysis and Multi-Experiment Analysis.
Allows higher throughput.
Expert results to Excel and Powerpoint
Supplied on a USB stick.
Easy-to-Use Interface
Pro 48 software uses a unique icon-driven user interface to simplify experimental design and setup.
Pre-set thermal profile defaults are provided for the most commonly used experimental protocols.
Temperature and time for each protocol step can easily be changed by click-and-drag action with the mouse.
Experiment templates can be customized and saved for future use.
Data Analysis
With the Pro 48 system and software, data collection is monitored in real time, allowing researchers to access run viability immediately.
The user-friendly data analysis interface also allows researchers to easily view the amplification plot, melt curve analysis, and the analysed results, including Cq values, PCR efficiency, R 2 and Y-intercept.
Data can be exported into Excel and custom reports generated directly into PowerPoint or PDF formats.
High-resolution images can be directly exported in multiple image formats, ready to use in presentations.
Conforms to Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, making data analysis and submission for publication review more efficient.
High-Performance Results
Due to unmatched temperature control and an optical system designed for individual well monitoring, the Pro 48 system produces highly accurate, reliable results with PCR efficiencies between 90% and 110% and R
2
> 0.99 when using standard optimized assays.
qPCR probe-based detection reagent kits
Every assay is expert designed, involving in-depth literature review
followed by extensive bioinformatics analysis. The kits are
optimised via a strict biochemical validation on synthetic oligos
to ensure optimal PCR performance. Every kit operates with the
same standardised protocol and cycling conditions, which allows
multiple kits to be used on the same plate in the same PCR run.
Designed and manufactured in the UK.
Wide range of 400 qPCR detection kits.
Designed to work with Pro 48 and all other
qPCR machines.
Kits sold with/without Mastermix, 150 tests.
Human Pathogens
Techne supply a comprehensive range of all the commonly tested infections and diseases. If your test is not available contact Techne or your local dealer to enquire about getting a custom kit made for you.
Respiratory Infections.
Sexually Transmitted Diseases.
Herpes Viral Infections.
Hepatitis Infections.
Human Papillomavirus.
Gastrointestinal Infections.
Vector-Borne Diseases.
Meningitis.
Periodontal Infections.
Biothreat detection
Techne supply a small but key list of detection kits, aimed at detecting some particularly harmful viruses and bacteria
Japanese Encephalitis virus.
Slow Bee Paralysis virus.
African Horse Sickness.
Taï Forest Ebola virus.
Sudan Ebola virus.
Rift Valley Fever virus.
Reston ebola virus.
Zaire ebola virus.
Bacillus anthracis.
Yersinia enterocolitica.
Food & Water Testing
With ever increasing demands for clarity on the source and type of products we are consuming, speciation and pathogen detection kits have been generated to allow users to do exactly that.
GMO.
Speciation.
Allergens.
Pathogen Contamination.
Veterinary and Agriculture
A wide range of veterinary and agricultural pathogen detection kits, all offering a potentially significant economic impact.
Avian.
Bovine.
Ovine
Caprine.
Equine.
Feline.
Canine.
Porcine.
Piscean.
In The Box
Ethernet cable
Power supply
USB Drive
Box of 50x Seals
Bag of 50x Plates
Dock
Manufacturer Specs
Techne Prime Pro 48 General Specifications
High Resolution Melt
Yes
Volume per well
Validated for 5 to 20 ul
Detection sensitivity
1 copy
Temp uniformity
+- 0.1°C
Temp accuracy
+- 0.15°C @ 60°C and 95°C
Temperature range
30 to 100 degC
Average ramp rate
5.5 degC sec
Thermal system
Hollow silver block peltier-based with conductive fluid
Block format
48-well block
Consumables
48-well custom plates and optical adhesive seals
Optical system
Dual LED excitation (452 to 486 nm and 542 to 582 nm)
Camera
CCD camera
Filters
4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)
Calibrated dyes
SYBR FAM HEX ROX Cy5
Additional dyes
All dyes within range compatible with no additional calibration
Passive reference dyes
Use of ROX is supported but optional
Data collection
Data always collected in all four filters for all wells
Plate setup
Plate setup for data analysis can be altered after run completes
Melt curve
Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
PCR cycle time (unoptimised)
40 cycles in less than 40 minutes
PCR cycle time (FAST optimised)
40 cycles in less than 20 minutes
Dynamic range
Greater than 9 logs
Calibration
Not required
Installation
Plug and play design
Precision
Discriminates 5000 and 10000 template copies with 99 percent confidence
Warranty
1 year (parts and labour included)
Voltage
100 to 240 V.AC
Frequency
50 and 60 Hz
Nominal current draw
5A
Peak power
500W (typical power is 180W)
Software
Multiple license software included at no additional cost
Chemistry
All chemistries supported
Applications
Absolute Quantification and Relative Quantification
Applications
Allelic Discrimination and High Resolution Melt
Dimensions closed (WxDxH)
34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)
Dimensions open (WxDxH)
34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)
Weight
13.6 Kg (30 lb)